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dc.contributor.authorMüller, Gerd A.
dc.contributor.authorQuaas, Marianne
dc.contributor.authorSchümann, Michael
dc.contributor.authorKrause, Eberhard
dc.contributor.authorPadi, Megha
dc.contributor.authorFischer, Martin
dc.contributor.authorLitovchick, Larisa
dc.contributor.authorDeCaprio, James A.
dc.contributor.authorEngeland, Kurt
dc.date.accessioned2012-08-03T11:29:25Z
dc.date.issued2011
dc.identifier.citationMüller, Gerd A., Marianne Quaas, Michael Schümann, Eberhard Krause, Megha Padi, Martin Fischer, Larisa Litovchick, James A. DeCaprio, and Kurt Engeland. 2011. The CHR promoter element controls cell cycle-dependent gene transcription and binds the DREAM and MMB complexes. Nucleic Acids Research 404(4): 1561-1578.en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:9361508
dc.description.abstractCell cycle-dependent gene expression is often controlled on the transcriptional level. Genes like \(cyclin B, CDC2\) and \(CDC25C\) are regulated by cell cycle-dependent element (CDE) and cell cycle genes homology region (CHR) promoter elements mainly through repression in \(G_0/G_1\). It had been suggested that E2F4 binding to CDE sites is central to transcriptional regulation. However, some promoters are only controlled by a CHR. We identify the DREAM complex binding to the CHR of mouse and human \(cyclin B2\) promoters in \(G_0\). Association of DREAM and cell cycle-dependent regulation is abrogated when the CHR is mutated. Although E2f4 is part of the complex, a CDE is not essential but can enhance binding of DREAM. We show that the CHR element is not only necessary for repression of gene transcription in \(G_0/G_1\), but also for activation in S, \(G_2\) and M phases. In proliferating cells, the B-myb-containing MMB complex binds the CHR of both promoters independently of the CDE. Bioinformatic analyses identify many genes which contain conserved CHR elements in promoters binding the DREAM complex. With \(Ube2c\) as an example from that screen, we show that inverse CHR sites are functional promoter elements that can bind DREAM and MMB. Our findings indicate that the CHR is central to DREAM/MMB-dependent transcriptional control during the cell cycle.en_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.isversionofdoi:10.1093/nar/gkr793en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287175/pdf/en_US
dash.licenseLAA
dc.titleThe CHR Promoter Element Controls Cell Cycle-Dependent Gene Transcription and Binds the DREAM and MMB Complexesen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalNucleic Acids Researchen_US
dash.depositing.authorLitovchick, Larisa
dc.date.available2012-08-03T11:29:25Z
dc.identifier.doi10.1093/nar/gkr793*
dash.contributor.affiliatedLitovchick, Larisa
dash.contributor.affiliatedPadi, Megha
dash.contributor.affiliatedDeCaprio, James


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