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dc.contributor.authorRitchie, Jennifer M.
dc.contributor.authorRui, Haopeng
dc.contributor.authorZhou, Xiaohui
dc.contributor.authorIida, Tetsuya
dc.contributor.authorKodoma, Toshio
dc.contributor.authorIto, Susuma
dc.contributor.authorDavis, Brigid
dc.contributor.authorBronson, Roderick Terry
dc.contributor.authorWaldor, Matthew K
dc.date.accessioned2012-08-10T17:23:34Z
dc.date.issued2012
dc.identifier.citationRitchie, Jennifer M., Haopeng Rui, Xiaohui Zhou, Tetsuya Iida, Toshio Kodoma, Susuma Ito, Brigid M. Davis, Roderick T. Bronson, and Matthew K. Waldor. 2012. Inflammation and disintegration of intestinal villi in an experimental model for \(Vibrio\) \(parahaemolyticus\)-induced diarrhea. PLoS Pathogens 8(3): e1002593.en_US
dc.identifier.issn1553-7366en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:9393134
dc.description.abstract\(Vibrio\) \(parahaemolyticus\) is a leading cause of seafood-borne gastroenteritis in many parts of the world, but there is limited knowledge of the pathogenesis of \(V.\) \(parahaemolyticus\)-induced diarrhea. The absence of an oral infection-based small animal model to study \(V.\) \(parahaemolyticus\) intestinal colonization and disease has constrained analyses of the course of infection and the factors that mediate it. Here, we demonstrate that infant rabbits oro-gastrically inoculated with \(V.\) \(parahaemolyticus\) develop severe diarrhea and enteritis, the main clinical and pathologic manifestations of disease in infected individuals. The pathogen principally colonizes the distal small intestine, and this colonization is dependent upon type III secretion system 2. The distal small intestine is also the major site of \(V.\) \(parahaemolyticus\)-induced tissue damage, reduced epithelial barrier function, and inflammation, suggesting that disease in this region of the gastrointestinal tract accounts for most of the diarrhea that accompanies \(V.\) \(parahaemolyticus\) infection. Infection appears to proceed through a characteristic sequence of steps that includes remarkable elongation of microvilli and the formation of \(V.\) \(parahaemolyticus\)-filled cavities within the epithelial surface, and culminates in villus disruption. Both depletion of epithelial cell cytoplasm and epithelial cell extrusion contribute to formation of the cavities in the epithelial surface. \(V.\) \(parahaemolyticus\) also induces proliferation of epithelial cells and recruitment of inflammatory cells, both of which occur before wide-spread damage to the epithelium is evident. Collectively, our findings suggest that \(V.\) \(parahaemolyticus\) damages the host intestine and elicits disease via previously undescribed processes and mechanisms.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.ppat.1002593en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3305451/pdf/en_US
dash.licenseLAA
dc.subjectbiologyen_US
dc.subjectmicrobiologyen_US
dc.subjectbacterial pathogensen_US
dc.subjectmedicineen_US
dc.subjectgastroenterology and hepatologyen_US
dc.subjectinfectious diseasesen_US
dc.titleInflammation and Disintegration of Intestinal Villi in an Experimental Model for \(Vibrio\) \(parahaemolyticus\)-Induced Diarrheaen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS Pathogensen_US
dash.depositing.authorZhou, Xiaohui
dc.date.available2012-08-10T17:23:34Z
dc.identifier.doi10.1371/journal.ppat.1002593*
dash.contributor.affiliatedZhou, Xiaohui
dash.contributor.affiliatedDavis, Brigid M.
dash.contributor.affiliatedWaldor, Matthew
dash.contributor.affiliatedBronson, Roderick


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