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dc.contributor.authorVijayan, Vikram
dc.contributor.authorJain, Isha Himani
dc.contributor.authorO'Shea, Erin K
dc.date.accessioned2012-10-22T17:58:33Z
dc.date.issued2012
dc.identifier.citationVijayan, Vikram, Isha H. Jain, and Erin K. O'Shea. 2012. A high resolution map of a cyanobacterial transcriptome. Genome Biology 12(5): R47.en_US
dc.identifier.issn1465-6906en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:9793865
dc.description.abstractBackground: Previous molecular and mechanistic studies have identified several principles of prokaryotic transcription, but less is known about the global transcriptional architecture of bacterial genomes. Here we perform a comprehensive study of a cyanobacterial transcriptome, that of Synechococcus elongatus PCC 7942, generated by combining three high-resolution data sets: RNA sequencing, tiling expression microarrays, and RNA polymerase chromatin immunoprecipitation sequencing. Results: We report absolute transcript levels, operon identification, and high-resolution mapping of 5' and 3' ends of transcripts. We identify several interesting features at promoters, within transcripts and in terminators relating to transcription initiation, elongation, and termination. Furthermore, we identify many putative non-coding transcripts. Conclusions: We provide a global analysis of a cyanobacterial transcriptome. Our results uncover insights that reinforce and extend the current views of bacterial transcription.en_US
dc.description.sponsorshipMolecular and Cellular Biologyen_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/gb-2011-12-5-r47en_US
dash.licenseOAP
dc.titleA High Resolution Map of a Cyanobacterial Transcriptomeen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalGenome Biologyen_US
dash.depositing.authorO'Shea, Erin K
dc.date.available2012-10-22T17:58:33Z
dc.identifier.doi10.1186/gb-2011-12-5-r47*
dash.contributor.affiliatedJain, Isha
dash.contributor.affiliatedVijayan, Vikram
dash.contributor.affiliatedO'Shea, Erin


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