Analysis of Inorganic Polyphosphates by Capillary Gel Electrophoresis

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Analysis of Inorganic Polyphosphates by Capillary Gel Electrophoresis

Show simple item record Lee, Andrew Whitesides, George M. 2012-11-06T17:37:54Z 2010
dc.identifier.citation Lee, Andrew and George M. Whitesides. 2010. Analysis of inorganic polyphosphates by capillary gel electrophoresis. 82(16): 6838–6846. en_US
dc.identifier.issn 0003-2700 en_US
dc.identifier.issn 1520-6882 en_US
dc.description.abstract This paper describes the development of a method that uses capillary gel electrophoresis (CGE) to analyze mixtures of inorganic polyphosphate ((P\(_i\))\(_n\)). Resolution of (P\(_i\))\(_n\) on the basis of n, the number of residues of dehydrated phosphate, is accomplished by CGE using capillaries filled with solutions of poly(N,N-dimethylacrylamide) (PDMA) and indirect detection by the UV absorbance of a chromophore, terephthalate, added to the running buffer. The method is capable of resolving peaks representing (P\(_i\))\(_n\) with n up to 70; preparation and use of authentic standards enables the identification of peaks for (P\(_i\))\(_n\) with n = 1−10. The main advantages of this method over previously reported methods for analyzing mixtures of (P\(_i\))\(_n\) (e.g., gel electrophoresis, CGE using polyacrylamide-filled capillaries) are its resolution, convenience, and reproducibility; gel-filled capillaries are easily regenerated by pumping in fresh, low-viscosity solutions of PDMA. The resolution is comparable to that of ion-exchange chromatography and detection of (P\(_i\))\(_n\) by suppressed conductivity. The method is useful for analyzing (P\(_i\))\(_n\) generated by the dehydration of P\(_i\) at low temperature (125−140 °C) with urea, in a reaction that may have been important in prebiotic chemistry. The method should also be useful for characterizing mixtures of other anionic, oligomeric, or polymeric species without an intrinsic chromophore (e.g., sulfated polysaccharides, oligomeric phospho-diesters). en_US
dc.description.sponsorship Chemistry and Chemical Biology en_US
dc.language.iso en_US en_US
dc.publisher American Chemical Society en_US
dc.relation.isversionof doi:10.1021/ac1008018 en_US
dc.relation.hasversion en_US
dc.relation.hasversion en_US
dash.license OAP
dc.subject replaceable linear polyacrylamide en_US
dc.subject nuclear-magnetic-resonance en_US
dc.subject catalyzed phosphorylation reactions en_US
dc.subject indirect photometric detection en_US
dc.subject single-stranded-DNA en_US
dc.subject condensed phosphates en_US
dc.subject zone-electrophoresis en_US
dc.subject polymer-solutions en_US
dc.subject quantitative analysis en_US
dc.subject chain polyphosphates en_US
dc.title Analysis of Inorganic Polyphosphates by Capillary Gel Electrophoresis en_US
dc.type Journal Article en_US
dc.description.version Author's Original en_US
dc.relation.journal Analytical Chemistry en_US Whitesides, George M. 2012-11-06T17:37:54Z

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