Kassis, Judith A.Noll, ElizabethVanSickle, E. PageOdenwald, Ward F.Perrimon, Norbert2021-10-041992-03-01Kassis, Judith A., Elizabeth Noll, E. Page VanSickle, Ward F. Odenwald, Norbert Perrimon. "Altering the Insertional Specificity of a Drosophila Transposable Element." Proceedings of the National Academy of Sciences 89, no. 5 (1992): 1919-1923. DOI: 10.1073/pnas.89.5.19190027-84241091-6490https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37369664Vectors derived from the Drosophila P element transposon are widely used to make transgenic Drosophila. Insertion of most P-element-derived vectors is nonrandom, but they exhibit a broad specificity of target sites. During experiments to identify cis-acting regulatory elements of the Drosophila segmentation gene engrailed, we identified a fragment of engrailed DNA that, when included within a P-element vector, strikingly alters the specificity of target sites. P-element vectors that contain this fragment of engrailed regulatory DNA insert at a high frequency near genes expressed in stripes.en-USJournal Article2021-10-0410.1073/pnas.89.5.1919