Chen, HaiqiLiu, SophiaPadula, SamuelLesman, DanielGriswold, KettnerLin, AllenZhao, TongtongMarshall, JamieChen, Fei2023-02-162019-12-16Chen, Haiqi, Sophia Liu, Samuel Padula, Daniel Lesman, Kettner Griswold, Allen Lin, Tongtong Zhao, Jamie L. Marshall, and Fei Chen. 2020. “Efficient, Continuous Mutagenesis in Human Cells Using a Pseudo-Random DNA Editor.” Nature Biotechnology 38 (2): 165–68.1087-01561546-1696https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37374385Here, we describe TRACE, a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. Using TRACE in a MEK1 inhibitor resistance screen, we identified functionally correlated mutations.en-USBiomedical EngineeringMolecular MedicineApplied Microbiology and BiotechnologyBioengineeringBiotechnologyJournal Article2023-02-1610.1038/s41587-019-0331-8