Saif, MariKwanten, Wilhelmus J.Carr, Jessica A.Chen, Ivy X.Posada, JessicaSrivastava, AmitabhZhang, JuanyeZheng, YiPinter, MatthiasChatterjee, SampurnaSoftic, SamirKahn, C.van Leyen, KlausBruns, Oliver T.Jain, RakeshBawendi, Moungi G.2021-10-082020-08Saif, Mari, Wilhelmus J. Kwanten, Jessica A. Carr, Ivy X. Chen, Jessica Posada, Amitabh Srivastava, Juanye Zhang et al. "Non-Invasive Monitoring of Chronic Liver Disease via Near-Infrared and Shortwave-Infrared Imaging of Endogenous Lipofuscin." Nature Biomedical Engineering 4, no. 8 (2020): 801-813. DOI: 10.1038/s41551-020-0569-y2157-846Xhttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37369729Monitoring the progression of non-alcoholic fatty liver disease is hindered by a lack of suitable non-invasive imaging methods. Here, we show that the endogenous pigment lipofuscin displays strong near-infrared and shortwave-infrared fluorescence when excited at 808 nm, enabling label-free imaging of liver injury in mice and the discrimination of pathological processes from normal liver processes with high specificity and sensitivity. We also show that the near-infrared and shortwave-infrared fluorescence of lipofuscin can be used to monitor the progression and regression of liver necroinflammation and fibrosis in mouse models of non-alcoholic fatty liver disease and advanced fibrosis, as well as to detect non-alcoholic steatohepatitis and cirrhosis in biopsied samples of human liver tissue.en-USResearch Subject Categories::MEDICINE::Physiology and pharmacology::Radiological research::Diagnostic radiologyJournal Article2021-10-0810.1038/s41551-020-0569-y