Alam, AshfaqulLaRocque, Regina C.Harris, Jason B.Vanderspurt, CecilyRyan, Edward T.Qadri, FirdausiCalderwood, Stephen B.2019-10-122005Alam, A., R. C. LaRocque, J. B. Harris, C. Vanderspurt, E. T. Ryan, F. Qadri, and S. B. Calderwood. 2005. “Hyperinfectivity of Human-Passaged Vibrio Cholerae Can Be Modeled by Growth in the Infant Mouse.” Infection and Immunity73 (10): 6674–79. doi:10.1128/IAI.73.10.6674-6679.2005.0019-95671070-63131098-5522http://nrs.harvard.edu/urn-3:HUL.InstRepos:41542714It has previously been shown that passage of Vibrio cholerae through the human intestine imparts a transient hyperinfectious phenotype that may contribute to the epidemic spread of cholera. The mechanism underlying this human-passaged hyperinfectivity is incompletely understood, in part due to inherent difficulties in recovering and studying organisms that are freshly passed in human stool. Here, we demonstrate that passage of V cholerae through the infant mouse intestine leads to an equivalent degree of hyperinfectivity as passage through the human host. We have used this infant mouse model of host-passaged hyperinfectivity to characterize the timing and the anatomic location of the competitive advantage of mouse-passaged V cholerae as well as the contribution of three type IV pili to the phenotype.en-USJournal Article2019-10-1210.1128/IAI.73.10.6674-6679.2005