Griffin, RuthSustar, AnneBonvin, MarianneBinari, Richarddel Valle Rodriguez, AlbertoHohl, Amber MBateman, Jack RVillalta, ChristiansHeffern, ElleardGrunwald, DidierBakal, ChrisDesplan, ClaudeSchubiger, GeroldWu, C-tingPerrimon, Norbert2021-03-222009-07-26Griffin, Ruth, Anne Sustar, Marianne Bonvin, Richard Binari, Alberto del Valle Rodriguez, Amber M. Hohl, Jack R. Bateman et al. 2009. "The twin spot generator for differential Drosophila lineage analysis." Nature Methods 6, no. 8: 600-602.1548-70911548-7105https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37367089In Drosophila, widely-used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the Twin Spot Generator technique (TSG) and demonstrate that through mitotic recombination, TSG generates green and red twin spots in internal fly tissues, visible even as single cells. We discuss the wide applications of TSG to lineage and genetic mosaic studies.en-USBiotechnologyCell BiologyBiochemistryMolecular BiologyJournal Article2021-03-2210.1038/nmeth.1349