Barrett, DianahLeimkuhler, CatherineChen, LanWalker, DeborahKahne, DanielWalker, Suzanne2019-10-032005Barrett, D., C. Leimkuhler, L. Chen, D. Walker, D. Kahne, and S. Walker. 2005. “Kinetic Characterization of the Glycosyltransferase Module of Staphylococcus Aureus PBP2.” Journal of Bacteriology 187 (6): 2215–17. https://doi.org/10.1128/jb.187.6.2215-2217.2005.0021-91931067-88321098-5530http://nrs.harvard.edu/urn-3:HUL.InstRepos:41467443We report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes.en-USJournal Article2019-10-0310.1128/JB.187.6.2215-2217.2005