Shun, Ming-ChiehDaigle, Janet E.Vandegraaff, NickEngelman, Alan2019-10-052007Shun, M.-C., J. E. Daigle, N. Vandegraaff, and A. Engelman. 2006. “Wild-Type Levels of Human Immunodeficiency Virus Type 1 Infectivity in the Absence of Cellular Emerin Protein.” Journal of Virology 81 (1): 166–72. https://doi.org/10.1128/jvi.01953-06.0022-538X1070-63211098-5514http://nrs.harvard.edu/urn-3:HUL.InstRepos:41482947Preintegration complexes (PICs) mediate retroviral integration, and recent results indicate an important role for the inner nuclear membrane protein emerin in orienting human immunodeficiency virus type 1 (HfV-1) PICs to chromatin for integration. Two other host cell proteins, the barrier-to-autointegration factor (BAF) and lamina-associated polypeptide 2 alpha (LAP2 alpha), seemed to play a similar preintegrative role for Moloney murine leukemia virus (MMLV) in addition to HIV-1. In contrast, we determined efficient HfV-1 and MMLV infection of HeLa-P4 cells following potent down-regulation of emerin, BAF, or LAP2 alpha protein by using short interfering RNA. Mouse embryo fibroblasts ablated for emerin protein through gene knockout support the same level of HIV-1 infection as cells derived from wild-type littermate control animals. As the expression of human emerin in mouse knockout cells fails to affect the level of infectivity achieved in its absence, we conclude that HIV-1 efficiently infects cells in the absence of emerin protein and, by extension, that emerin is not a universally important regulator of HIV-1 infectivity.en-USJournal Article2019-10-0510.1128/JVI.01953-06