Cherry, SaraDoukas, TammyArmknecht, SusanWhelan, SeanWang, HuiSarnow, PeterPerrimon, Norbert2021-10-062005-02-15Cherry, Sara, Tammy Doukas, Susan Armknecht, Sean Whelan, Hui Wang, Peter Sarnow, Norbert Perrimon. "Genome-Wide RNAi Screen Reveals a Specific Sensitivity of IRES-Containing RNA Viruses to Host Translation Inhibition." Genes and Development 19, no. 4 (2005): 445-452. DOI: 10.1101/gad.12679050890-93691549-5477https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37369715The widespread class of RNA viruses that utilize internal ribosome entry sites (IRESs) for translation include poliovirus and Hepatitis C virus. To identify host factors required for IRES-dependent translation and viral replication, we performed a genome-wide RNAi screen in Drosophila cells infected with Drosophila C virus (DCV). We identified 66 ribosomal proteins that, when depleted, specifically inhibit DCV growth, but not a non-IRES-containing RNA virus. Moreover, treatment of flies with a translation inhibitor is protective in vivo. Finally, this increased sensitivity to ribosome levels also holds true for poliovirus infection of human cells, demonstrating the generality of these findings.en-USJournal Article2021-10-0610.1101/gad.1267905