Kapitzky, LauraBeltrao, PedroBerens, Theresa J.Gassner, NadineZhou, ChunshuiWüster, ArthurWu, JulieBabu, M. MadanElledge, Stephen J.Toczyski, DavidLokey, R. ScottKrogan, Nevan J.2019-10-122010Kapitzky, Laura, Pedro Beltrao, Theresa J Berens, Nadine Gassner, Chunshui Zhou, Arthur Wüster, Julie Wu, et al. 2010. “Cross‐species Chemogenomic Profiling Reveals Evolutionarily Conserved Drug Mode of Action.” Molecular Systems Biology 6 (1): 451. https://doi.org/10.1038/msb.2010.107.1744-4292http://nrs.harvard.edu/urn-3:HUL.InstRepos:41542679We present a cross-species chemogenomic screening platform using libraries of haploid deletion mutants from two yeast species, Saccharomyces cerevisiae and Schizosaccharomyces pombe. We screened a set of compounds of known and unknown mode of action (MoA) and derived quantitative drug scores (or D-scores), identifying mutants that are either sensitive or resistant to particular compounds. We found that compound-functional module relationships are more conserved than individual compound-gene interactions between these two species. Furthermore, we observed that combining data from both species allows for more accurate prediction of MoA. Finally, using this platform, we identified a novel small molecule that acts as a DNA damaging agent and demonstrate that its MoA is conserved in human cells. Molecular Systems Biology 6: 451; published online 21 December 2010; doi:10.1038/msb.2010.107en-USJournal Article2019-10-1210.1038/msb.2010.107