Ratnapriya, SnehaChov, AngelaHerschhorn, Alon2023-05-182020-12-18Ratnapriya, Sneha, Angela Chov, and Alon Herschhorn. 2020. “A Protocol for Studying HIV-1 Envelope Glycoprotein Function.” STAR Protocols 1 (3): 100133–100133. https://doi.org/10.1016/j.xpro.2020.100133.2666-1667https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37375235HIV-1 envelope glycoproteins (Envs) bind to CD4 receptor and CCR5/CXCR4 coreceptor and mediate viral entry (Feng et al., 1996; Herschhorn et al., 2016, 2017; Kwong et al., 1998). HIV-1 Envs are the sole target of neutralizing antibodies and a main focus of vaccine development (Flemming et al., 2018). Here, we provide a step-by-step protocol to measure Env sensitivity to ligands, cold, and small molecules, as well as to study viral infectivity and to dissect parameters affecting HIV-1 Env function. For complete details on the use and execution of this protocol, please refer to Harris et al. (2020).en-USGeneral Immunology and MicrobiologyGeneral Biochemistry, Genetics and Molecular BiologyGeneral NeuroscienceJournal Article2023-05-1810.1016/j.xpro.2020.100133