Sütterlin, ChristineLin, Chin-YoFeng, YangFerris, Douglas K.Erikson, Raymond L.Malhotra, Vivek2019-10-112001Sutterlin, C., C.-Y. Lin, Y. Feng, D. K. Ferris, R. L. Erikson, and V. Malhotra. 2001. “Polo-like Kinase Is Required for the Fragmentation of Pericentriolar Golgi Stacks during Mitosis.” Proceedings of the National Academy of Sciences98 (16): 9128–32. https://doi.org/10.1073/pnas.161283998.0027-84240744-28311091-6490http://nrs.harvard.edu/urn-3:HUL.InstRepos:41534407The pericentriolar stacks of Golgi cisternae undergo extensive reorganization during mitosis in mammalian cells. GM130 and GRASP65 (Golgi reassembly stacking protein of 65 kDa) are Golgi-associated proteins that are targets of mitotic kinases, and they have also been implicated in the reorganization of the Golgi structure during cell division. Previous studies have reported that mitogen-activated protein kinase kinase-1 (MEK1} and Cdc2 protein kinases are involved in these dynamic changes in the Golgi structure. More recently, the mitotic polo-like kinase (Plk} has been shown to interact with and phosphorylate GRASP65. Here, we provide evidence that Plk is involved in the mitosis-specific fragmentation of the Golgi apparatus. The addition of kinase-defective Plk or immunodepletion of Plk disrupts the fragmentation process. Furthermore, Golgi fragmentation is inhibited by the addition of either full-length or truncated GRASP65. These findings suggest that phospharylation of GRASP65 by Plk may be a critical event in the reorganization of the Golgi structure during mitosis.en-USJournal Article2019-10-1110.1073/pnas.161283998