Kulczyk, ArkadiuszMoeller, ArneMeyer, PeterSliz, PiotrRichardson, Charles2019-10-052017Kulczyk, Arkadiusz W., Arne Moeller, Peter Meyer, Piotr Sliz, and Charles C. Richardson. 2017. “Cryo-EM Structure of the Replisome Reveals Multiple Interactions Coordinating DNA Synthesis.” Proceedings of the National Academy of Sciences 114 (10): E1848–56. https://doi.org/10.1073/pnas.1701252114.0027-84240744-28311091-6490http://nrs.harvard.edu/urn-3:HUL.InstRepos:41483401We present a structure of the similar to 650-kDa functional replisome of bacteriophage T7 assembled on DNA resembling a replication fork. A structure of the complex consisting of six domains of DNA helicase, five domains of RNA primase, two DNA polymerases, and two thioredoxin (processivity factor) molecules was determined by single-particle cryo-electron microscopy. The two molecules of DNA polymerase adopt a different spatial arrangement at the replication fork, reflecting their roles in leading-and lagging-strand synthesis. The structure, in combination with biochemical data, reveals molecular mechanisms for coordination of leading-and lagging-strand synthesis. Because mechanisms of DNA replication are highly conserved, the observations are relevant to other replication systems.en-USJournal Article2019-10-0510.1073/pnas.1701252114