Saxena, VishalOrgill, DennisKohane, Isaac2012-02-042006Saxena, Vishal, Dennis Orgill, and Isaac Kohane. 2006. Absolute enrichment: gene set enrichment analysis for homeostatic systems. Nucleic Acids Research 34, no. 22: e151.0305-1048http://nrs.harvard.edu/urn-3:HUL.InstRepos:8123170The Gene Set Enrichment Analysis (GSEA) identifies sets of genes that are differentially regulated in one direction. Many homeostatic systems will include one limb that is upregulated in response to a downregulation of another limb and vice versa. Such patterns are poorly captured by the standard formulation of GSEA. We describe a technique to identify groups of genes (which sometimes can be pathways) that include both up- and down-regulated components. This approach lends insights into the feedback mechanisms that may operate, especially when integrated with protein interaction databases.en-USJournal Article2012-02-0410.1093/nar/gkl766