Levin, Joshua ZBerger, Michael FAdiconis, XianRogov, PeterMelnikov, AlexandreFennell, TimothyNusbaum, ChadGarraway, LeviGnirke, Andreas2012-04-082009Levin, Joshua Z., Michael F. Berger, Xian Adiconis, Peter Rogov, Alexandre Melnikov, Timothy Fennell, Chad Nusbaum, Levi A. Garraway, and Andreas Gnirke. 2009. Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts. Genome Biology 10(10): R115.1465-6906http://nrs.harvard.edu/urn-3:HUL.InstRepos:8519632Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes.en-USJournal Article2012-04-0810.1186/gb-2009-10-10-r115