Antipova, Alena A.Stockwell, Brent R.Golub, Todd2012-12-112008Antipova, Alena A., Brent R. Stockwell, and Todd R. Golub. 2008. Gene expression-based screening for inhibitors of PDGFR signaling. Genome Biology 9(3): R47.1465-6906http://nrs.harvard.edu/urn-3:HUL.InstRepos:10024401Here we describe a proof-of-concept experiment designed to explore the possibility of using gene expression-based high-throughput screening (GE-HTS) to find inhibitors of a signaling cascade, using platelet derived growth factor receptor (PDGFR) signaling as the example. The previously unrecognized ability of aurintricarboxylic acid to inhibit PDGFR signaling, discovered through a screen of 1,739 compounds, demonstrates the feasibility and generalizability of GE-HTS for the discovery of small molecule modulators of any signaling pathway of interest.en-USchronic myelomonocytic leukemiacolony-stimulating factoraurintricarboxylic acidgrowth factorpotent inhibitorreceptoractivationproteinmechanismJournal Article2012-12-1110.1186/gb-2008-9-3-r47