Chen, YuzhiBodles, Angela MMcPhie, DonnaNeve, Rachael LMrak, Robert EGriffin, W Sue T2011-02-202007Chen, Yuzhi, Angela M. Bodles, Donna L. McPhie, Rachael L. Neve, Robert E. Mrak, and W. Sue T. Griffin. 2007. APP-BP1 inhibits Aβ42 levels by interacting with Presenilin-1. Molecular Neurodegeneration 2: 3.1750-1326http://nrs.harvard.edu/urn-3:HUL.InstRepos:4728485Background: The β-amyloid precursor protein (APP) is sequentially cleaved by the β- and then γ-secretase to generate the amyloid β-peptides Aβ40 and Aβ42. Increased Aβ42/Aβ40 ratios trigger amyloid plaque formations in Alzheimer's disease (AD). APP binds to APP-BP1, but the biological consequence is not well understood. Results: We report that when the endogenous APP-BP1 was suppressed by small interfering RNAs (siRNAs), cell-associated Aβ42 was dramatically increased in APP695 expressing primary neurons. The accumulation of Aβ42 was accompanied by significant increases in APP and APP-CTF in APP-BP1 siRNA expressing neurons. In contrast, APP-BP1 overexpression in primary neurons significantly decreased the levels of Aβ and endogenous APP but not APLPs. We also investigated the potential mechanism of APP-BP1-mediated APP processing. APP-BP1 co-precipitated with Presenilin-1 (PS1) in native rat brain extracts, co-migrated with the γ-secretase components in brain membrane extracts in glycerol gradient centrifugation, and colocalized in primary neurons. Further, the endogenous PS1-CTF was significantly downregulated by APP-BP1 expression. Conclusion: Our data suggest that APP-BP1 may inhibit Aβ42 production by interacting with PS1 under physiological conditions.en-USJournal Article2011-02-2010.1186/1750-1326-2-3