Kim, Richard DPark, Peter2013-01-302004Kim, Richard D., and Peter J. Park. 2004. Improving identification of differentially expressed genes in microarray studies using information from public databases. Genome Biology 5(9): R70.1465-6906http://nrs.harvard.edu/urn-3:HUL.InstRepos:10246812Summary: The process of identifying differentially expressed genes in miroarray studies with small sample sizes can be improved substantially by extracting information from a large number of datasets accumulated in public databases. Abstract: We demonstrate that the process of identifying differentially expressed genes in microarray studies with small sample sizes can be substantially improved by extracting information from a large number of datasets accumulated in public databases. The improvement comes from more reliable estimates of gene-specific variances based on other datasets. For a two-group comparison with two arrays in each group, for example, the result of our method was comparable to that of a t-test analysis with five samples in each group or to that of a regularized t-test analysis with three samples in each group. Our results are further improved by weighting the results of our approach with the regularized t-test results in a hybrid method.en-USJournal Article2013-01-3010.1186/gb-2004-5-9-r70