de Chassey, BNavratil, VTafforeau, LHiet, M SAublin-Gex, AAgaugué, SMeiffren, GPradezynski, FFaria, B FChantier, TLe Breton, MPellet, JDavoust, NMangeot, P EChaboud, APenin, FJacob, YVidalain, P OVidal, MarcAndré, PRabourdin-Combe, CLotteau, V2010-12-212008de Chassey, B., V. Navratil, L. Tafforeau, M. S. Hiet, A. Aublin-Gex, S. Agaugué, G. Meiffren, et al. 2008. Hepatitis C virus infection protein network. Molecular Systems Biology 4: 230.1744-4292http://nrs.harvard.edu/urn-3:HUL.InstRepos:4632528A proteome-wide mapping of interactions between hepatitis C virus (HCV) and human proteins was performed to provide a comprehensive view of the cellular infection. A total of 314 protein–protein interactions between HCV and human proteins was identified by yeast two-hybrid and 170 by literature mining. Integration of this data set into a reconstructed human interactome showed that cellular proteins interacting with HCV are enriched in highly central and interconnected proteins. A global analysis on the basis of functional annotation highlighted the enrichment of cellular pathways targeted by HCV. A network of proteins associated with frequent clinical disorders of chronically infected patients was constructed by connecting the insulin, Jak/STAT and TGFβ pathways with cellular proteins targeted by HCV. CORE protein appeared as a major perturbator of this network. Focal adhesion was identified as a new function affected by HCV, mainly by NS3 and NS5A proteins.en-USfunctional analysishepatitis Cinteractomevirus–host cellJournal Article2010-12-2110.1038/msb.2008.66