Lundequist, AndersBoyce, Joshua2011-09-292011Lundequist, Anders, and Joshua A. Boyce. 2011. LPA5 Is abundantly expressed by human mast cells and important for lysophosphatidic acid induced MIP-1β release. PLoS ONE 6(3): e18192.1932-6203http://nrs.harvard.edu/urn-3:HUL.InstRepos:5146965Background: Lysophosphatidic acid (LPA) is a bioactive lipid inducing proliferation, differentiation as well as cytokine release by mast cells through G-protein coupled receptors. Recently GPR92/LPA5 was identified as an LPA receptor highly expressed by cells of the immune system, which prompted us to investigate its presence and influence on mast cells. Principal Findings: Transcript analysis using quantitative real-time PCR revealed that LPA5 is the most prevalent LPA-receptor in human mast cells. Reduction of LPA5 levels using shRNA reduced calcium flux and abolished MIP-1β release in response to LPA. Conclusions: LPA5 is a bona fide LPA receptor on human mast cells responsible for the majority of LPA induced MIP-1β release.en-USbiochemistrylipid mediatorsproteinsimmune system proteinstransmembrane proteinsgeneticsgene expressionimmunologymembrane receptor signalingsignal transductionclinical immunologyimmunologic receptor signalingG-protein signalingJournal Article2011-09-2910.1371/journal.pone.0018192