Kim, Ju HeeKang, SunghyunJung, Suk-KyeongYu, Keum RanChung, Sang J.Chung, Bong HyunErikson, RaymondKim, Bo YeonKim, Seung Jun2014-02-212012Kim, Ju Hee, Sunghyun Kang, Suk-Kyeong Jung, Keum Ran Yu, Sang J. Chung, Bong Hyun Chung, Raymond L. Erikson, Bo Yeon Kim, and Seung Jun Kim. 2012. Crystal structure of xenotropic murine leukaemia virus-related virus (xmrv) ribonuclease h. Bioscience Reports 32(Pt 5): 455-463.0144-8463http://nrs.harvard.edu/urn-3:HUL.InstRepos:11729584RNase H (retroviral ribonuclease H) cleaves the phosphate backbone of the RNA template within an RNA/DNA hybrid to complete the synthesis of double-stranded viral DNA. In the present study we have determined the complete structure of the RNase H domain from XMRV (xenotropic murine leukaemia virus-related virus) RT (reverse transcriptase). The basic protrusion motif of the XMRV RNase H domain is folded as a short helix and an adjacent highly bent loop. Structural superposition and subsequent mutagenesis experiments suggest that the basic protrusion motif plays a role in direct binding to the major groove in RNA/DNA hybrid, as well as in establishing the co-ordination among modules in RT necessary for proper function.en-USS2murine leukaemia virusreverse transcriptaseribonuclease Hxenotropic murine leukaemia virus-related virus (XMRV)DTT, dithiothreitolMLV, murine leukaemia virusMoMLV, Moloney murine leukaemia virus-related virusRNase H, retroviral ribonuclease Hrms, root-mean-squareRT, reverse transcriptaseXMRV, xenotropic murine leukaemia virus-related virusJournal Article2014-02-2110.1042/BSR20120028