Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment

McGregor, Lynn MarieGorin, David JoelDumelin, ChristophLiu, David2013-10-242010McGregor, Lynn M., David J. Gorin, Christoph E. Dumelin, and David R. Liu. 2010. Interaction-Dependent PCR: Identification of Ligandâ Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment. Journal of the American Chemical Society 132(44): 15522-15524.0002-7863http://nrs.harvard.edu/urn-3:HUL.InstRepos:11211555Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein−ligand pairs out of 67 599 possible sequences.en-USInteraction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase ExperimentJournal Article2013-10-2410.1021/ja107677q