Mattiazzi, M.Jambhekar, AshwiniKaferle, P.DeRisi, J. L.Križaj, I.Petrovič, U.2011-04-232010Mattiazzi, M., A. Jambhekar, P. Kaferle, J. L. DeRisi, I. Križaj, and U. Petrovič. 2010. Genetic interactions between a phospholipase A2 and the Rim101 pathway components in S. cerevisiae reveal a role for this pathway in response to changes in membrane composition and shape. Molecular Genetics and Genomics 283(6): 519-530.1617-4615http://nrs.harvard.edu/urn-3:HUL.InstRepos:4874830Modulating composition and shape of biological membranes is an emerging mode of regulation of cellular processes. We investigated the global effects that such perturbations have on a model eukaryotic cell. Phospholipases A[sub]2 (PLA[sub]2s), enzymes that cleave one fatty acid molecule from membrane phospholipids, exert their biological activities through affecting both membrane composition and shape. We have conducted a genome-wide analysis of cellular effects of a PLA[sub]2 in the yeast Saccharomyces cerevisiae as a model system. We demonstrate functional genetic and biochemical interactions between PLA[sub]2 activity and the Rim101 signaling pathway in S. cerevisiae. Our results suggest that the composition and/or the shape of the endosomal membrane affect the Rim101 pathway. We describe a genetically and functionally related network, consisting of components of the Rim101 pathway and the prefoldin, retromer and SWR1 complexes, and predict its functional relation to PLA[sub]2 activity in a model eukaryotic cell. This study provides a list of the players involved in the global response to changes in membrane composition and shape in a model eukaryotic cell, and further studies are needed to understand the precise molecular mechanisms connecting them.Electronic supplementary material The online version of this article (doi:10.1007/s00438-010-0533-8) contains supplementary material, which is available to authorized users.en-USRim101 pathwayPhospholipase A2endosomal membranegenetic interactionsJournal Article2011-04-2310.1007/s00438-010-0533-8