Jacobs, Pieter P.Inan, MehmetFestjens, NeleHaustraete, JurgenVan Hecke, AnneliesContreras, RolandMeagher, Michael MCallewaert, Nico2011-04-282010Jacobs, Pieter P., Mehmet Inan, Nele Festjens, Jurgen Haustraete, Annelies Van Hecke, Roland Contreras, Michael M. Meagher, and Nico Callewaert. 2010. Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate. Microbial Cell Factories 9: 93.1475-2859http://nrs.harvard.edu/urn-3:HUL.InstRepos:4878941Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce. Results: Here, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at high levels (hundreds of milligrams per liter). We demonstrate that homogenous Man5GlcNAc2 N-glycosylation of the secreted proteins is achieved at all specific growth rates tested. Conclusions: Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins.en-USJournal Article2011-04-2810.1186/1475-2859-9-93