Lang, Gregory I.Botstein, DavidDesai, Michael2013-10-032011Lang, Gregory I., David Botstein, and Michael M. Desai. Genetic variation and the fate of individual beneficial mutations in asexual populations. 2011. Genetics 188:647-661.0016-6731http://nrs.harvard.edu/urn-3:HUL.InstRepos:11130442The fate of a newly arising beneficial mutation depends on many factors, such as the population size and the availability and fitness effects of other mutations that accumulate in the population. It has proven difficult to understand how these factors influence the trajectories of particular mutations, since experiments have primarily focused on characterizing successful clones emerging from a small number of evolving populations. Here, we present the results of a massively parallel experimental designed to measure the full spectrum of possible fates of new beneficial mutations in hundreds of experimental yeast populations, whether these mutations are ultimately successful or not. Using strains in which a particular class of beneficial mutation is detectable by fluorescence, we followed the trajectories of these beneficial mutations across 592 independent populations for 1,000 generations. We find that the fitness advantage provided by individual mutations plays a surprisingly small role. Rather, underlying “background” genetic variation is quickly generated in our initially clonal populations and plays a crucial role in determining the fate of each individual beneficial mutation in the evolving population.en-USexperimental evolutionclonal interferenceadaptationasexual evolutionJournal Article2013-10-0310.1534/genetics.111.128942