Qin, YuSchneider, TobiasBrenner, Michael2013-10-242012Qin, Yu, Tobias M. Schneider, and Michael P. Brenner. 2012. Sequencing by hybridization of long targets. PLoS ONE 7(5): e35819.1932-6203http://nrs.harvard.edu/urn-3:HUL.InstRepos:11210605Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined \(l\)-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to \(n=O(2^{l})\) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error.en-USbiologycomputational biologygenomicsgenome sequencingsequence analysisgenome analysis toolssequence assembly toolstheoretical biologymathematicsapplied mathematicsalgorithmsJournal Article2013-10-2410.1371/journal.pone.0035819