Simonis, NicolasRual, Jean-FrançoisLemmens, IrmaBoxus, MathieuHirozane-Kishikawa, TomokoGatot, Jean-StéphaneDricot, AmélieHao, TongVertommen, DidierLegros, SébastienDaakour, SarahKlitgord, NielsMartin, MaudWillaert, Jean-FrançoisDequiedt, FranckNavratil, VincentBurny, ArsèneVan Lint, CarineTavernier, JanKettmann, RichardTwizere, Jean-ClaudeCusick, MichaelHill, DavidVidal, Marc2013-03-082012Simonis, Nicolas, Jean-François Rual, Irma Lemmens, Mathieu Boxus, Tomoko Hirozane-Kishikawa, Jean-Stéphane Gatot, Amélie Dricot, et al. 2012. Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses. Retrovirology 9:26.1742-4690http://nrs.harvard.edu/urn-3:HUL.InstRepos:10381380Background: Human T-cell leukemia virus type 1 (HTLV-1) and type 2 both target T lymphocytes, yet induce radically different phenotypic outcomes. HTLV-1 is a causative agent of Adult T-cell leukemia (ATL), whereas HTLV-2, highly similar to HTLV-1, causes no known overt disease. HTLV gene products are engaged in a dynamic struggle of activating and antagonistic interactions with host cells. Investigations focused on one or a few genes have identified several human factors interacting with HTLV viral proteins. Most of the available interaction data concern the highly investigated HTLV-1 Tax protein. Identifying shared and distinct host-pathogen protein interaction profiles for these two viruses would enlighten how they exploit distinctive or common strategies to subvert cellular pathways toward disease progression. Results: We employ a scalable methodology for the systematic mapping and comparison of pathogen-host protein interactions that includes stringent yeast two-hybrid screening and systematic retest, as well as two independent validations through an additional protein interaction detection method and a functional transactivation assay. The final data set contained 166 interactions between 10 viral proteins and 122 human proteins. Among the 166 interactions identified, 87 and 79 involved HTLV-1 and HTLV-2 -encoded proteins, respectively. Targets for HTLV-1 and HTLV-2 proteins implicate a diverse set of cellular processes including the ubiquitin-proteasome system, the apoptosis, different cancer pathways and the Notch signaling pathway. Conclusions: This study constitutes a first pass, with homogeneous data, at comparative analysis of host targets for HTLV-1 and -2 retroviruses, complements currently existing data for formulation of systems biology models of retroviral induced diseases and presents new insights on biological pathways involved in retroviral infection.en-USHTLVinteractomeretrovirusORFeometaxHBZJournal Article2013-03-0810.1186/1742-4690-9-26