Wu, JunShekhar, NandiniLele, Pushkar PrakashLele, Tanmay P.2014-02-202012Wu, Jun, Nandini Shekhar, Pushkar P. Lele, and Tanmay P. Lele. 2012. Frap analysis: accounting for bleaching during image capture. PLoS ONE 7(8): e42854.1932-6203http://nrs.harvard.edu/urn-3:HUL.InstRepos:11726260The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleaching during image capture. In this paper, we explicitly include the effects of bleaching during image capture in the model for the recovery process, instead of correcting for the effects of bleaching using reference measurements. Using experimental examples, we demonstrate the usefulness of such an approach in FRAP analysis.en-USBiologyBiochemistryProteinsProtein ChemistryBiophysicsBiophysics TheoryMacromolecular AssembliesDevelopmental BiologyMolecular DevelopmentAdhesion MoleculesMolecular Cell BiologyCell AdhesionSystems BiologyChemistryPhysical ChemistryChemical DynamicsEngineeringBioengineeringBiological Systems EngineeringPhysicsJournal Article2014-02-2010.1371/journal.pone.0042854