Lechene, ClaudeLee, GloriaPoczatek, J. CollinToner, MehmetBiggers, John2013-03-292012Lechene, Claude P., Gloria Y. Lee, J. Collin Poczatek, Mehmet Toner, and John D. Biggers. 2012. 3D multi-isotope imaging mass spectrometry reveals penetration of \(^{18}O\)-trehalose in mouse sperm nucleus. PLoS ONE 7(8): e42267.1932-6203http://nrs.harvard.edu/urn-3:HUL.InstRepos:10482571The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permitting tolerance to desiccation. These results have broad implications for long-term storage of mammalian cells.en-USBiologyAnatomy and PhysiologyCell PhysiologyReproductive SystemBiotechnologyGenetic EngineeringTransgenicsCryobiologyDevelopmental BiologyMolecular DevelopmentHistologyModel OrganismsAnimal ModelsMouseJournal Article2013-03-2910.1371/journal.pone.0042267