Sigoillot, Frederic D.Lyman, SusanHuckins, Jeremy F.Adamson, Brittany SusanChung, EunahQuattrochi, BrianKing, Randall2013-04-222012Sigoillot, Frederic D., Susan Lyman, Jeremy F. Huckins, Britt Adamson, Eunah Chung, Brian Quattrochi, and Randall W. King. 2012. A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens. Nature Methods 9(4): 363-366.1548-70911548-7105http://nrs.harvard.edu/urn-3:HUL.InstRepos:10579115Because off-target effects hamper interpretation and validation of RNAi screens, we developed a bioinformatics method, Genome-wide Enrichment of Seed Sequence matches (GESS), to identify candidate off-targeted transcripts from direct analysis of primary screening data. GESS identified a prominent off-targeted transcript in several screens, including MAD2 in a screen for components of the spindle assembly checkpoint. We demonstrate how incorporation of the results of GESS analysis can enhance the validation rate in RNAi screens.en-USJournal Article2013-04-2210.1038/nmeth.1898