Seidman, Jonathan G.Seidman, Christine EdryChristodoulou, Danos2013-10-082013-10-082013Christodoulou, Danos C. 2013. Methods for comprehensive transcriptome analysis using next-generation sequencing and application in hypertrophic cardiomyopathy. Doctoral dissertation, Harvard University.http://dissertations.umi.com/gsas.harvard:10749http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156672Characterization of the RNA transcriptome by next-generation sequencing can produce an unprecedented yield of information that provides novel biologic insights. I describe four approaches for sequencing different aspects of the transcriptome and provide computational tools to analyze the resulting data. Methods that query the dynamic range of gene expression, low expressing transcripts, micro RNA levels, and start-site usage of transcripts are described.en-USGeneticsfhl1genetic modifierhypertrophic cardiomyopathyinherited cardiomyopathiesnext-generation sequencingrna-seqThesis or Dissertation2013-10-08