Church, George McDonaldMosberg, Joshua Adam Weintrob2013-10-082013-10-082013Mosberg, Joshua Adam Weintrob. 2013. Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli. Doctoral dissertation, Harvard University.http://dissertations.umi.com/gsas.harvard:10777http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156813The phage-derived Lambda Red recombination system utilizes exogenous DNA in order to generate precise insertion, deletion, and point mutations in Escherichia coli and other bacteria. Due to its convenience, it is a frequently-used tool in genetics and molecular biology, as well as in larger-scale genome engineering projects. However, limited recombination frequency constrains the usefulness of Lambda Red for several important applications. In this work, I utilize a mechanism-guided approach in order to improve the power and utility of Lambda Red recombination.en-USBiologyGeneticsMolecular biologyLambdaNucleasePrimaseRecombinationRecombineeringRedThesis or Dissertation2014-06-07