Lu, FakeJi, MinbiaoFu, DanNi, XiaohuiFreudiger, Christian WilhelmHoltom, GaryXie, Xiaoliang2013-07-162012Lu, Fa-Ke, Minbiao Ji, Dan Fu, Xiaohui Ni, Christian W. Freudiger, Gary Holtom, and X. Sunney Xie. 2012. Multicolor stimulated Raman scattering microscopy. Molecular Physics 110(15-16): 1927-1932.0026-8976http://nrs.harvard.edu/urn-3:HUL.InstRepos:10875724Stimulated Raman scattering (SRS) microscopy has opened up a wide range of biochemical imaging applications by probing a particular Raman-active molecule vibrational mode in the specimen. However, the original implementation with picosecond pulse excitation can only realize rapid chemical mapping with a single Raman band. Here we present a novel SRS microscopic technique using a grating-based pulse shaper for excitation and a grating-based spectrograph for detection to achieve simultaneous multicolor SRS imaging with high sensitivity and high acquisition speeds. In particular, we use a linear combination of the measured \(CH_2\) and \(CH_3\) stretching signals to map the distributions of protein and lipid contents simultaneously.en-UScoherent Raman scatteringstimulated Raman scatteringmulticolorgratingpulse shaperlock-inpolystyrenepoly(methyl methacrylate)mouse skinproteinlipidJournal Article2013-07-1610.1080/00268976.2012.695028