Chetty, SundariPagliuca, FeliciaHonore, ChristianKweudjeu, AnastasieRezania, AlirezaMelton, Douglas2017-06-092013Chetty, Sundari, Felicia Walton Pagliuca, Christian Honore, Anastasie Kweudjeu, Alireza Rezania, and Douglas A Melton. 2013. “A Simple Tool to Improve Pluripotent Stem Cell Differentiation.” Nature Methods 10 (6) (April 14): 553–556. doi:10.1038/nmeth.2442. http://dx.doi.org/10.1038/nmeth.2442.1548-7091http://nrs.harvard.edu/urn-3:HUL.InstRepos:32969831We describe a method to help overcome restrictions on the differentiation propensities of human pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle and, in more than 25 embryonic and induced pluripotent stem cell lines, improves directed differentiation into multiple lineages. DMSO treatment also improves differentiation into terminal cell types in several cell lines.en-USJournal Article2014-02-07Chetty S, Pagliuca F, Honore C, Kweudjeu A, Rezania A, Melton DA10.1038/nmeth.2442