Szostak, Jack WilliamBlain, Jonathan Craig2014-02-252014-02-252013Blain, Jonathan Craig. 2013. Non-Enzymatic Copying of Nucleic Acid Templates. Doctoral dissertation, Harvard University.http://dissertations.umi.com/gsas.harvard:11219http://nrs.harvard.edu/urn-3:HUL.InstRepos:11744416All known living cells contain a complex set of molecular machinery to support their growth and replication. However, the earliest cells must have been much simpler, consisting of a compartment and a genetic material to allow for Darwinian evolution. To study these intermediates, plausible model `protocells' must be synthesized in the laboratory since no fossils remain. Recent work has shown that fatty acids can self-assemble into vesicles that are able to grow and divide through simple mechanisms. However, a self-replicating protocell genome has not yet been developed. Here we discuss studies of systems that allow for the copying of nucleic acid templates without enzymes and how they could be developed into a genetic material.en-USGeneticsBiochemistryChemistryClick chemistryOrigin of lifeProtocellSelf replicationSynthetic biologyThesis or Dissertation2016-02-04