Church, George McDonaldLajoie, Marc2014-02-252014-02-252014Lajoie, Marc Joseph. 2014. Genome Engineering Technologies to Change the Genetic Code. Doctoral dissertation, Harvard University.http://dissertations.umi.com/gsas.harvard:11265http://nrs.harvard.edu/urn-3:HUL.InstRepos:11745697New technologies are making it possible to engineer organisms with fundamentally new and useful properties. In vivo genome engineering technologies capable of manipulating genomes from the nucleotide to the megabase scale were developed and applied to reassign the genetic code of Escherichia coli. Such genomically recoded organisms show promise for thwarting horizontal gene transfer with natural organisms, resisting viral infection, and expanding the chemical properties of proteins.en-USBiologyGenetic codeGenetic isolationGenome engineeringMAGERecombinationVirus resistanceThesis or Dissertation2014-02-25