Freudiger, Christian WilhelmYang, WenlongHoltom, GaryPeyghambarian, NasserXie, XiaoliangKieu, Khanh Q.2014-02-282014Freudiger, Christian W., Wenlong Yang, Gary R. Holtom, Nasser Peyghambarian, X. Sunney Xie, and Khanh Q. Kieu. 2014. “Stimulated Raman Scattering Microscopy with a Robust Fibre Laser Source.” Nature Photon 8 (2) (January 19): 153–159. doi:10.1038/nphoton.2013.360. http://dx.doi.org/10.1038/nphoton.2013.360.1749-4885http://nrs.harvard.edu/urn-3:HUL.InstRepos:11831705Stimulated Raman scattering microscopy allows label-free chemical imaging and has enabled exciting applications in biology, material science and medicine. It provides a major advantage in imaging speed over spontaneous Raman scattering and has improved image contrast and spectral fidelity compared to coherent anti-Stokes Raman scattering. Wider adoption of the technique has, however, been hindered by the need for a costly and environmentally sensitive tunable ultrafast dual-wavelength source. We present the development of an optimized all-fibre laser system based on the optical synchronization of two picosecond power amplifiers. To circumvent the high-frequency laser noise intrinsic to amplified fibre lasers, we have further developed a high-speed noise cancellation system based on voltage-subtraction autobalanced detection. We demonstrate uncompromised imaging performance of our fibre-laser-based stimulated Raman scattering microscope with shot-noise-limited sensitivity and an imaging speed up to 1 frame \(s^{−1}\).en-USJournal Article2013-12-03Christian W. Freudiger, Wenlong Yang, Gary R. Holtom, Nasser Peyghambarian, X. Sunney Xie, Khanh Q. Kieu2014-07-2110.1038/nphoton.2013.360