Shiroto, TakashiRomero, NataliaSugiyama, ToruSartoretto, Juliano L.Kalwa, HermannYan, ZhonghuaShimokawa, HiroakiMichel, Thomas2014-03-112014Shiroto, Takashi, Natalia Romero, Toru Sugiyama, Juliano L. Sartoretto, Hermann Kalwa, Zhonghua Yan, Hiroaki Shimokawa, and Thomas Michel. 2014. “Caveolin-1 Is a Critical Determinant of Autophagy, Metabolic Switching, and Oxidative Stress in Vascular Endothelium.” PLoS ONE 9 (2): e87871. doi:10.1371/journal.pone.0087871. http://dx.doi.org/10.1371/journal.pone.0087871.1932-6203http://nrs.harvard.edu/urn-3:HUL.InstRepos:11879746Caveolin-1 is a scaffolding/regulatory protein that interacts with diverse signaling molecules. Caveolin-1null mice have marked metabolic abnormalities, yet the underlying molecular mechanisms are incompletely understood. We found the redox stress plasma biomarker plasma 8-isoprostane was elevated in caveolin-1null mice, and discovered that siRNA-mediated caveolin-1 knockdown in endothelial cells promoted significant increases in intracellular H2O2. Mitochondrial ROS production was increased in endothelial cells after caveolin-1 knockdown; 2-deoxy-D-glucose attenuated this increase, implicating caveolin-1 in control of glycolytic pathways. We performed unbiased metabolomic characterizations of endothelial cell lysates following caveolin-1 knockdown, and discovered strikingly increased levels (up to 30-fold) of cellular dipeptides, consistent with autophagy activation. Metabolomic analyses revealed that caveolin-1 knockdown led to a decrease in glycolytic intermediates, accompanied by an increase in fatty acids, suggesting a metabolic switch. Taken together, these results establish that caveolin-1 plays a central role in regulation of oxidative stress, metabolic switching, and autophagy in the endothelium, and may represent a critical target in cardiovascular diseases.en-USBiologyBiochemistryMetabolismMetabolic PathwaysModel OrganismsAnimal ModelsMouseMolecular Cell BiologyCellular TypesEndothelial CellsSignal TransductionSignaling in Cellular ProcessesRedox SignalingMedicineCardiovascularCardiovascular PharmacologyVascular BiologyJournal Article2014-03-1110.1371/journal.pone.0087871