Ran, ChongzhaoZhang, ZhaodaHooker, JacobMoore, Anna2017-07-202011Ran, Chongzhao, Zhaoda Zhang, Jacob Hooker, and Anna Moore. 2011. “In Vivo Photoactivation Without ‘Light’: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light.” Molecular Imaging and Biology 14 (2) (May 3): 156–162. doi:10.1007/s11307-011-0489-z.1536-1632http://nrs.harvard.edu/urn-3:HUL.InstRepos:33471162Purpose: The poor tissue penetration of visible light has been a major barrier for optical imaging, photoactivatable conversions, and photodynamic therapy for in vivo targets with depths beyond 10 mm. In this report, as a proof-of-concept, we demonstrated that a positron emission tomography (PET) radiotracer, 2-deoxy-2-[18F]fluoro-d-glucose (18FDG), could be used as an alternative light source for photoactivation. Procedures: We utilized 18FDG, which is a metabolic activity-based PET probe, as a source of light to photoactivate caged luciferin in a breast cancer animal model expressing luciferase. Results: Bioluminescence produced from luciferin allowed for the real-time monitoring of Cherenkov radiation-promoted uncaging of the substrate. Conclusion: The proposed method may provide a very important option for in vivo photoactivation, in particular for activation of photosensitizers for photodynamic therapy and eventually for combining radioisotope therapy and photodynamic therapy.en-USJournal Article10.1007/s11307-011-0489-z