Vella, Sarah J.Beattie, PatrickCademartiri, RebeccaLaromaine, AnnaMartinez, Andres W.Phillips, Scott T.Mirica, Katherine A.Whitesides, George2014-03-202012Vella, Sarah J., Patrick Beattie, Rebecca Cademartiri, Anna Laromaine, Andres W. Martinez, Scott T. Phillips, Katherine A. Mirica, and George M. Whitesides. 2012. Measuring Markers of Liver Function Using a Micropatterned Paper Device Designed for Blood from a Fingerstick. Analytical Chemistry 84, no. 6: 2883–2891.0003-27001520-6882http://nrs.harvard.edu/urn-3:HUL.InstRepos:11956907This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and “spiked” standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.en-USJournal Article2014-03-2010.1021/ac203434x