Zhang, ChenJang, SunyoungAmadi, Ovid C.Shimizu, KoichiLee, RichardMitchell, Richard2014-03-102013Zhang, Chen, Sunyoung Jang, Ovid C. Amadi, Koichi Shimizu, Richard T. Lee, and Richard N. Mitchell. 2013. “A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device.” BioMed Research International 2013 (1): 373569. doi:10.1155/2013/373569. http://dx.doi.org/10.1155/2013/373569.2314-6133http://nrs.harvard.edu/urn-3:HUL.InstRepos:11878819Existing chemotaxis assays do not generate stable chemotactic gradients and thus—over time—functionally measure only nonspecific random motion (chemokinesis). In comparison, microfluidic technology has the capacity to generate a tightly controlled microenvironment that can be stably maintained for extended periods of time and is, therefore, amenable to adaptation for assaying chemotaxis. We describe here a novel microfluidic device for sensitive assay of cellular migration and show its application for evaluating the chemotaxis of smooth muscle cells in a chemokine gradient.en-USJournal Article2014-03-1010.1155/2013/373569