Sompallae, RamakrishnaHofmann, OliverMaher, Christopher A.Gedye, CraigBehren, AndreasVitezic, MoranaDaub, Carsten O.Devalle, SylvieCaballero, Otavia L.Carninci, PieroHayashizaki, YoshihideLawlor, Elizabeth R.Cebon, JonathanHide, Winston2014-03-112013Sompallae, R., O. Hofmann, C. A. Maher, C. Gedye, A. Behren, M. Vitezic, C. O. Daub, et al. 2013. “A comprehensive promoter landscape identifies a novel promoter for CD133 in restricted tissues, cancers, and stem cells.” Frontiers in Genetics 4 (1): 209. doi:10.3389/fgene.2013.00209. http://dx.doi.org/10.3389/fgene.2013.00209.1664-8021http://nrs.harvard.edu/urn-3:HUL.InstRepos:11879030PROM1 is the gene encoding prominin-1 or CD133, an important cell surface marker for the isolation of both normal and cancer stem cells. PROM1 transcripts initiate at a range of transcription start sites (TSS) associated with distinct tissue and cancer expression profiles. Using high resolution Cap Analysis of Gene Expression (CAGE) sequencing we characterize TSS utilization across a broad range of normal and developmental tissues. We identify a novel proximal promoter (P6) within CD133+ melanoma cell lines and stem cells. Additional exon array sampling finds P6 to be active in populations enriched for mesenchyme, neural stem cells and within CD133+ enriched Ewing sarcomas. The P6 promoter is enriched with respect to previously characterized PROM1 promoters for a HMGI/Y (HMGA1) family transcription factor binding site motif and exhibits different epigenetic modifications relative to the canonical promoter region of PROM1.en-USPROM1 proteinhumanAC133 antigentranscription start sitepromoter regionsgeneticmelanomacancer stem cellsJournal Article2014-03-1110.3389/fgene.2013.00209