Jiang, LinWallerman, OlaYounis, ShadyRubin, Carl-JohanGilbert, Elizabeth R.Sundström, ElisabethGhazal, AwaisaZhang, XiaolanWang, LiMikkelsen, Tarjei S.Andersson, GöranAndersson, Leif2014-05-062014Jiang, L., O. Wallerman, S. Younis, C. Rubin, E. R. Gilbert, E. Sundström, A. Ghazal, et al. 2014. “ZBED6 Modulates the Transcription of Myogenic Genes in Mouse Myoblast Cells.” PLoS ONE 9 (4): e94187. doi:10.1371/journal.pone.0094187. http://dx.doi.org/10.1371/journal.pone.0094187.1932-6203http://nrs.harvard.edu/urn-3:HUL.InstRepos:12152887ZBED6 is a recently discovered transcription factor, unique to placental mammals, that has evolved from a domesticated DNA transposon. It acts as a repressor at the IGF2 locus. Here we show that ZBED6 acts as a transcriptional modulator in mouse myoblast cells, where more than 700 genes were differentially expressed after Zbed6-silencing. The most significantly enriched GO term was muscle protein and contractile fiber, which was consistent with increased myotube formation. Twenty small nucleolar RNAs all showed increased expression after Zbed6-silencing. The co-localization of histone marks and ZBED6 binding sites and the effect of Zbed6-silencing on distribution of histone marks was evaluated by ChIP-seq analysis. There was a strong association between ZBED6 binding sites and the H3K4me3, H3K4me2 and H3K27ac modifications, which are usually found at active promoters, but no association with the repressive mark H3K27me3. Zbed6-silencing led to increased enrichment of active marks at myogenic genes, in agreement with the RNA-seq findings. We propose that ZBED6 preferentially binds to active promoters and modulates transcriptional activity without recruiting repressive histone modifications.en-USBiology and life sciencesCell BiologyChromosome BiologyChromatinComputational BiologyGenome AnalysisTranscriptome AnalysisGenome Expression AnalysisGeneticsEpigeneticsHistone modificationRNA interferenceGene expressionDNA transcriptionGene regulationGenomicsFunctional GenomicsMolecular GeneticsJournal Article2014-05-0610.1371/journal.pone.0094187