Cheng, Christine S.Rai, KunalGarber, ManuelHollinger, AndrewRobbins, DanaAnderson, ScottMacbeth, AlyssaTzou, AustinCarneiro, Mauricio O.Raychowdhury, RaktimaRuss, CarstenHacohen, NirGershenwald, Jeffrey E.Lennon, NiallNusbaum, ChadChin, LyndaRegev, AvivAmit, Ido2014-05-062013Cheng, C. S., K. Rai, M. Garber, A. Hollinger, D. Robbins, S. Anderson, A. Macbeth, et al. 2013. “Semiconductor-based DNA sequencing of histone modification states.” Nature communications 4 (1): 2672. doi:10.1038/ncomms3672. http://dx.doi.org/10.1038/ncomms3672.2041-1723http://nrs.harvard.edu/urn-3:HUL.InstRepos:12152958The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.en-USJournal Article2014-05-0610.1038/ncomms3672