Qi, HaoGhodousi, MajidDu, YananGrun, CaseyBae, HojaeYin, PengKhademhosseini, Ali2014-04-112013Qi, Hao, Majid Ghodousi, Yanan Du, Casey Grun, Hojae Bae, Peng Yin, and Ali Khademhosseini. 2013. “DNA directed self-assembly of shape-controlled hydrogels.” Nature communications 4 (1): 2275. doi:10.1038/ncomms3275. http://dx.doi.org/10.1038/ncomms3275.2041-1723http://nrs.harvard.edu/urn-3:HUL.InstRepos:12064445Using DNA as programmable, sequence specific ‘glues’, shape-controlled hydrogel units are self-assembled into prescribed structures. Here we report that aggregates are produced using hydrogel cubes with edge length ranging from 30 micrometers to 1 millimeter, demonstrating assembly across scales. In a simple one-pot agitation reaction, 25 dimers are constructed in parallel from 50 distinct hydrogel cube species, demonstrating highly multiplexed assembly. Using hydrogel cuboids displaying face-specific DNA glues, diverse structures are achieved in aqueous and in interfacial agitation systems. These include dimers, extended chains, and open network structures in an aqueous system; and dimers, chains of fixed length, T-junctions, and square shapes in the interfacial system, demonstrating the versatility of the assembly system.en-USJournal Article2014-04-1110.1038/ncomms3275