Nizza, Suzanne T.Campbell, James J.2014-04-112014Nizza, Suzanne T., and James J. Campbell. 2014. “CD11b+ Migratory Dendritic Cells Mediate CD8 T Cell Cross-Priming and Cutaneous Imprinting after Topical Immunization.” PLoS ONE 9 (3): e91054. doi:10.1371/journal.pone.0091054. http://dx.doi.org/10.1371/journal.pone.0091054.1932-6203http://nrs.harvard.edu/urn-3:HUL.InstRepos:12064449Topical antigen application is a focus of current vaccine research. This immunization route mimics natural antigen exposure across a barrier tissue and generates T cells imprinted for skin-selective homing. Soluble antigens introduced through this route require cross-presentation by DC to generate CD8 T cell responses. Here we have explored the relative contribution of various skin-derived DC subsets to cross-priming and skin-selective imprinting. In our model, DC acquire soluble Ag in vivo from immunized murine skin for cross-presentation to naïve CD8 T cells ex vivo. We find CD11b+ migratory DC to be the relevant cross-priming DC in this model. Both Langerin+ and Langerin- CD11b+ migratory DC can cross-present antigen in our system, but only the Langerin+ subset can induce expression of the skin-selective addressin E-selectin ligand. Thus, the CD11b+ Langerin+ migratory DC population, comprised primarily of Langerhans cells, both cross-primes naïve CD8 T cells and imprints them with skin-homing capabilities.en-USBiologyImmunologyImmune CellsAntigen-Presenting CellsT CellsImmune SystemLymphoid OrgansImmunityImmune ActivationAntigen Processing and RecognitionImmune ResponseImmunologic TechniquesMedicineClinical ImmunologyJournal Article2014-04-1110.1371/journal.pone.0091054