Jeong, Jee-YeongZhou, Jin-RongGao, ChongFeldman, LaurieSytkowski, Arthur J.2014-10-012014Jeong, Jee-Yeong, Jin-Rong Zhou, Chong Gao, Laurie Feldman, and Arthur J. Sytkowski. 2014. “Human selenium binding protein-1 (hSP56) is a negative regulator of HIF-1α and suppresses the malignant characteristics of prostate cancer cells.” BMB Reports 47 (7): 411-416. doi:10.5483/BMBRep.2014.47.7.104. http://dx.doi.org/10.5483/BMBRep.2014.47.7.104.1976-6696http://nrs.harvard.edu/urn-3:HUL.InstRepos:12987331In the present study, we demonstrate that ectopic expression of 56-kDa human selenium binding protein-1 (hSP56) in PC-3 cells that do not normally express hSP56 results in a marked inhibition of cell growth in vitro and in vivo. Down-regulation of hSP56 in LNCaP cells that normally express hSP56 results in enhanced anchorage-independent growth. PC-3 cells expressing hSP56 exhibit a significant reduction of hypoxia inducible protein (HIF)-1α protein levels under hypoxic conditions without altering HIF-1α mRNA (HIF1A) levels. Taken together, our findings strongly suggest that hSP56 plays a critical role in prostate cells by mechanisms including negative regulation of HIF-1α, thus identifying hSP56 as a candidate anti-oncogene product. [BMB Reports 2014; 47(7): 411-416]en-USHypoxia inducible factorProstate cancerProtein-protein interactionpVHL-interacting deubiquitinating enzymeSelenium binding proteinJournal Article2014-10-0110.5483/BMBRep.2014.47.7.104